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picrosirius red staining kit  (Servicebio Inc)

 
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    Structured Review

    Servicebio Inc picrosirius red staining kit
    LOX inhibitor improves MIA-induced joint damage (A) Representative images of H&E staining of rat joint tissues across four groups. Scale bars, 200 μm, n = 4. (B) <t>Picrosirius</t> red staining images of rat joint tissues across four groups. Scale bars, 100 μm. (C–F) Immunohistochemistry of LOX, IL-1β, MFAP5, and FBN1, rat joint tissues across four groups. IOD, integrated optical density. Scale bars, 400 μm, n = 4. Data were presented as mean ± SEM. Statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons test (A and C–F). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
    Picrosirius Red Staining Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/picrosirius+red/pmc13276305-41-0-5?v=Servicebio+Inc
    Average 86 stars, based on 1 article reviews
    picrosirius red staining kit - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "MFAP5 + synovial fibroblasts drive LOX upregulation to promote osteoarthritis progression"

    Article Title: MFAP5 + synovial fibroblasts drive LOX upregulation to promote osteoarthritis progression

    Journal: iScience

    doi: 10.1016/j.isci.2026.116286

    LOX inhibitor improves MIA-induced joint damage (A) Representative images of H&E staining of rat joint tissues across four groups. Scale bars, 200 μm, n = 4. (B) Picrosirius red staining images of rat joint tissues across four groups. Scale bars, 100 μm. (C–F) Immunohistochemistry of LOX, IL-1β, MFAP5, and FBN1, rat joint tissues across four groups. IOD, integrated optical density. Scale bars, 400 μm, n = 4. Data were presented as mean ± SEM. Statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons test (A and C–F). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
    Figure Legend Snippet: LOX inhibitor improves MIA-induced joint damage (A) Representative images of H&E staining of rat joint tissues across four groups. Scale bars, 200 μm, n = 4. (B) Picrosirius red staining images of rat joint tissues across four groups. Scale bars, 100 μm. (C–F) Immunohistochemistry of LOX, IL-1β, MFAP5, and FBN1, rat joint tissues across four groups. IOD, integrated optical density. Scale bars, 400 μm, n = 4. Data were presented as mean ± SEM. Statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons test (A and C–F). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Techniques Used: Staining, Immunohistochemistry



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    LOX inhibitor improves MIA-induced joint damage (A) Representative images of H&E staining of rat joint tissues across four groups. Scale bars, 200 μm, n = 4. (B) <t>Picrosirius</t> red staining images of rat joint tissues across four groups. Scale bars, 100 μm. (C–F) Immunohistochemistry of LOX, IL-1β, MFAP5, and FBN1, rat joint tissues across four groups. IOD, integrated optical density. Scale bars, 400 μm, n = 4. Data were presented as mean ± SEM. Statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons test (A and C–F). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    LOX inhibitor improves MIA-induced joint damage (A) Representative images of H&E staining of rat joint tissues across four groups. Scale bars, 200 μm, n = 4. (B) <t>Picrosirius</t> red staining images of rat joint tissues across four groups. Scale bars, 100 μm. (C–F) Immunohistochemistry of LOX, IL-1β, MFAP5, and FBN1, rat joint tissues across four groups. IOD, integrated optical density. Scale bars, 400 μm, n = 4. Data were presented as mean ± SEM. Statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons test (A and C–F). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    LOX inhibitor improves MIA-induced joint damage (A) Representative images of H&E staining of rat joint tissues across four groups. Scale bars, 200 μm, n = 4. (B) <t>Picrosirius</t> red staining images of rat joint tissues across four groups. Scale bars, 100 μm. (C–F) Immunohistochemistry of LOX, IL-1β, MFAP5, and FBN1, rat joint tissues across four groups. IOD, integrated optical density. Scale bars, 400 μm, n = 4. Data were presented as mean ± SEM. Statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons test (A and C–F). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    (A) Schematic representation of the tamoxifen-inducible Col1a1 KO mouse model. Created with Biorender.com . (B) Mean tumor growth curves of wildtype littermate control and non-tamoxifen-treated Col1a1 KO mice. (C) Col1a1 gene expression in tumors from control and non-tamoxifen-treated Col1a1 KO mice. (B-C) n = 7-8 mice per group. (D) Mean tumor growth curves of tamoxifen-treated control and Col1a1 KO mice. (E) Col1a1 gene expression in tumors from tamoxifen-treated control and Col1a1 KO mice. (D-E) n = 8-9 mice per group. (F-G) Serum levels of the collagen formation product PRO-C1 (F) and the collagen degradation product C1M (G) from tamoxifen-treated control and Col1a1 KO mice. Some samples were below the lower limit of detection (2.2ng/mL for PRO-C1 and 6.38 for C1M). (H-I) Quantification (left) and representative images (right) of <t>picrosirius</t> red (PSR) (H) and Masson’s Trichrome (I) staining of Pan02 tumors from tamoxifen-treated control and Col1a1 KO mice. Each data point represents the average of 10 random squares per image. Statistical significance was assessed using the two-tailed student’s t-test (C, E-I) and two-way ANOVA with Bonferroni correction (D) . Bars represent mean ± SEM (B,D) or SD (C, E-I) . * p <0.05, ** p <0.01, *** p <0.001.
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    Image Search Results


    LOX inhibitor improves MIA-induced joint damage (A) Representative images of H&E staining of rat joint tissues across four groups. Scale bars, 200 μm, n = 4. (B) Picrosirius red staining images of rat joint tissues across four groups. Scale bars, 100 μm. (C–F) Immunohistochemistry of LOX, IL-1β, MFAP5, and FBN1, rat joint tissues across four groups. IOD, integrated optical density. Scale bars, 400 μm, n = 4. Data were presented as mean ± SEM. Statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons test (A and C–F). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: iScience

    Article Title: MFAP5 + synovial fibroblasts drive LOX upregulation to promote osteoarthritis progression

    doi: 10.1016/j.isci.2026.116286

    Figure Lengend Snippet: LOX inhibitor improves MIA-induced joint damage (A) Representative images of H&E staining of rat joint tissues across four groups. Scale bars, 200 μm, n = 4. (B) Picrosirius red staining images of rat joint tissues across four groups. Scale bars, 100 μm. (C–F) Immunohistochemistry of LOX, IL-1β, MFAP5, and FBN1, rat joint tissues across four groups. IOD, integrated optical density. Scale bars, 400 μm, n = 4. Data were presented as mean ± SEM. Statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons test (A and C–F). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: Picrosirius red staining Kit , Servicebio , Cat# G1078.

    Techniques: Staining, Immunohistochemistry

    (A) Schematic representation of the tamoxifen-inducible Col1a1 KO mouse model. Created with Biorender.com . (B) Mean tumor growth curves of wildtype littermate control and non-tamoxifen-treated Col1a1 KO mice. (C) Col1a1 gene expression in tumors from control and non-tamoxifen-treated Col1a1 KO mice. (B-C) n = 7-8 mice per group. (D) Mean tumor growth curves of tamoxifen-treated control and Col1a1 KO mice. (E) Col1a1 gene expression in tumors from tamoxifen-treated control and Col1a1 KO mice. (D-E) n = 8-9 mice per group. (F-G) Serum levels of the collagen formation product PRO-C1 (F) and the collagen degradation product C1M (G) from tamoxifen-treated control and Col1a1 KO mice. Some samples were below the lower limit of detection (2.2ng/mL for PRO-C1 and 6.38 for C1M). (H-I) Quantification (left) and representative images (right) of picrosirius red (PSR) (H) and Masson’s Trichrome (I) staining of Pan02 tumors from tamoxifen-treated control and Col1a1 KO mice. Each data point represents the average of 10 random squares per image. Statistical significance was assessed using the two-tailed student’s t-test (C, E-I) and two-way ANOVA with Bonferroni correction (D) . Bars represent mean ± SEM (B,D) or SD (C, E-I) . * p <0.05, ** p <0.01, *** p <0.001.

    Journal: bioRxiv

    Article Title: Collagen type I promotes pancreatic tumor growth and limits immune cell infiltration

    doi: 10.64898/2026.01.09.698616

    Figure Lengend Snippet: (A) Schematic representation of the tamoxifen-inducible Col1a1 KO mouse model. Created with Biorender.com . (B) Mean tumor growth curves of wildtype littermate control and non-tamoxifen-treated Col1a1 KO mice. (C) Col1a1 gene expression in tumors from control and non-tamoxifen-treated Col1a1 KO mice. (B-C) n = 7-8 mice per group. (D) Mean tumor growth curves of tamoxifen-treated control and Col1a1 KO mice. (E) Col1a1 gene expression in tumors from tamoxifen-treated control and Col1a1 KO mice. (D-E) n = 8-9 mice per group. (F-G) Serum levels of the collagen formation product PRO-C1 (F) and the collagen degradation product C1M (G) from tamoxifen-treated control and Col1a1 KO mice. Some samples were below the lower limit of detection (2.2ng/mL for PRO-C1 and 6.38 for C1M). (H-I) Quantification (left) and representative images (right) of picrosirius red (PSR) (H) and Masson’s Trichrome (I) staining of Pan02 tumors from tamoxifen-treated control and Col1a1 KO mice. Each data point represents the average of 10 random squares per image. Statistical significance was assessed using the two-tailed student’s t-test (C, E-I) and two-way ANOVA with Bonferroni correction (D) . Bars represent mean ± SEM (B,D) or SD (C, E-I) . * p <0.05, ** p <0.01, *** p <0.001.

    Article Snippet: Sections were stained with picrosirius red (Ampliqon) according to manufacturer’s instructions or Masson’s trichrome according to manufacturer’s instructions.

    Techniques: Control, Gene Expression, Staining, Two Tailed Test